Sexing Goat Embryos by PCR Amplification of X- and Y- chromosome Specific Sequence of the Amelogenin Gene
نویسندگان
چکیده
منابع مشابه
Sexing Goat Embryos by PCR Amplification of X- and Y- chromosome Specific Sequence of the Amelogenin Gene*
The objective of this study was to develop a simplified, efficient, and accurate protocol for sexing goat embryos. Based on the amelogenin gene located on the conservation region of Xand Ychromosomes, a pair of primers was utilized and the system of PCR was established to amplify a 262 bp fragment from the Xchromosome in female goats, and a 262 bp fragment from Xchromosome and 202 bp fragment f...
متن کاملIdentification of sex-specific polymorphic sequences in the goat amelogenin gene for embryo sexing.
Amelogenin (AMEL) is a conserved gene located on the sex chromosomes of mammals. It is involved in the formation of enamel, which is the hard, white material that forms the protective outer layer of each tooth. In this study, we first cloned and determined the intron sequences of the goat AMELX and AMELY genes from female and male ear tissues. The polymorphic AMEL alleles were further analyzed ...
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ژورنال
عنوان ژورنال: Asian-Australasian Journal of Animal Sciences
سال: 2007
ISSN: 1011-2367,1976-5517
DOI: 10.5713/ajas.2007.1689